Design and application of guide device for femoral neck section / 中国骨伤

OBJECTIVE@#To explore the application effect of a new guide device designed according to the anatomical characteristics of the femoral neck cross section to assist the internal fixation of multiple screw of the femoral neck.@*METHODS@#From October to December 2016, 10 adult dry femur specimens, including 7 males and 3 females, aged 37 to 58(47.5±7.5) years old, were selected. Three hollow screws were implanted to simulate the treatment of femoral neck fracture with the new guider technology and the traditional guider technology as the control. The screw location accuracy parameters, screw puncture times, screw parallelism, operation time and fluoroscopy times of the two methods were recorded and compared.@*RESULTS@#Sixty screws were successfully implanted in 20 specimens. There was no significant difference in screw parallelism, operation time and fluoroscopy times between the two methods(>0.05). There were significant differences in the distance between screw and cortex, the distance between screw and femoral neck, the area ratio between screw and femoral neck, the distance between screws and the number of screw punctures between two methods(<0.05). It showed that the new type of guide had more advantages than the traditional method in locating screw accuracy and reducing the number of punctures.@*CONCLUSIONS@#Compared with the traditional technique, the new guider and percutaneous puncture technique under two-dimensional fluoroscopy have better localization of internal fixation screw for femoral neck fracture.

Discerning the femoral neck anteversion (FNA) from the torsion angle on 3D CT / 中国骨伤

<p><b>OBJECTIVE</b>To discern the differences between femoral neck anteversion (FNA) and torsion angle through 3D CT reconstruction.</p><p><b>METHODS</b>From March 2010 to October 2010,30 healthy adult volunteers' femur were reconstructed by 3D CT, included 15 males and 15 females with an average age of (43.66 +/- 7.57) years old ranging from 25 to 65 years. Display the FNA and the torsion angle by image post-processing, measuring torsion angle by "Center way" and direct measurement of FNA.</p><p><b>RESULTS</b>FNA was the angle between the axle wire of femoral neck and the shape face of femoral,the angle were (13.326 +/- 6.085) degrees. Torsion angle was the angle between the macropinacoid of cross section of femoral neck and the shape face of femoral, the angle were(31.335 +/- 2.079) degrees. There was no significant difference in left and right femur.</p><p><b>CONCLUSION</b>FNA is different from torsion angle. FNA is the angle between the line and the surface with the sharp angle towards the lower outside. The torsion angle is the angle between the two surfaces with the sharp angle towards the lower back.</p>

Expression of Yes-associated protein in non-small cell lung cancer and its relationship with clinical pathological factors / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Yes-associated protein (YAP) plays an important role in signal transduction and gene transcription regulation in normal cells, with elevated and over-expressed YAP levels observed in various malignant tumors. The aim of this study was to investigate the expression of YAP in non-small cell lung cancer (NSCLC), and to study the possible relationship of YAP expression with the occurrence and development of NSCLC.</p><p><b>METHODS</b>YAP expression was assessed in 40 cases of NSCLC tumor tissues by immunohistochemistry, and their protein and mRNA levels were evaluated through Western blotting and reverse transcription-polymerase chain reaction (PCR), respectively. Normal lung tissues obtained from the same patient were used as control. Statistical analysis was performed to correlate the YAP expression to clinical pathological factors, such as tumor type, stage and grade.</p><p><b>RESULTS</b>YAP-positive expression was found in 28 (70%) of the 40 cases of NSCLC, which included 10 cases of squamous cell carcinoma (25%), 17 cases of adenocarcinoma (42.5%) and 1 case of squamous adenocarcinoma (2.5%). In the 28 YAP-positive cases, 19 cases showed lymph node metastasis and were classified in TNM stage II + III (47.5%); the other nine cases showed no lymph node metastasis (22.5%) and were classified in the TNM stage I. There was no relationship between YAP expression and patients' age, gender or tumor histological grades. However, YAP showed significant over expression in late period of T stage (P = 0.012), TNM stage (P = 0.039), and lymph node metastasis (P = 0.013), respectively. Notably, YAP-positive expression was significantly higher in adenocarcinoma than that in squamous cell carcinoma (P = 0.041).</p><p><b>CONCLUSIONS</b>Over-expression of YAP was associated with NSCLC, especially lung adenocarcinoma. The high YAP expression in late period of tumor stage and lymph node metastasis may indicate that YAP expression could be an early marker for NSCLC tumorigenesis.</p>

Isolation and Purification of Exopolysaccharide from the Fermentation Broth of Bacillus sp. and Its Antioxidant Effect / 微生物学通报

Based on the Bacillus sp., isolated from Lop Nur Desert, the technology of separation and purification and the antioxidant effect were studied. After centrifugation and vacuum filtration, the deproteinization of supernatant was operated with Sevag reagent. The crude exopolysaccharide (EPS) was obtained by precipitation with ethanol. The optimum conditions for the isolation were as follow: pH 7.0, temperature 4?C, time 1.5 h, and material to ethanol ratio 1: 4. Dissolved in water, the crude EPS was fractional separated on activated carbon column (1.5 cm ? 24 cm), eluted with distilled water, 60% ethanol, 95% ethanol, and the main fraction was collected. Then the EPS was purified on Sephadex G-100 gel column, eluted with NaCl (0.2 mol/L). Fractions (4 mL, each) were also combined according to total sugar by phenol-sulfuric acid method and protein content was determined by Coomassie brilliant blue. The results showed that EPS was relatively homogeneous glycoprotein. The data of antioxidation in vitro showed that the EPS had a high antioxidant activity, which could quench hydroxyl radical, superoxide radical and had antilipid peroxidation activity. All of these indicated that EPS was a good natural antioxidant.